| Species | human |
| Individual | patient |
| Cell type | cancer cell |
| Cancer type | breast |
| Age | 42 |
| Sex | female |
| TNM Status | T4N1M0 |
| Status at the analysis timepoint | non-metastatic |
| Moment of samples collection | preoperative |
| Clinical material | peripheral blood |
| Collection | EDTA tubes |
| Fixative/storage | 4% PFA/-80̊ C |
| Protocol | pan-keratin (K) / vimentin (V) / DAPI / α-SMA / CD29 / CD45/CD31 |
| acquisition conditions | Objective 40x, low flow, lasers/power: 405 nm/100 mW, 488 nm /100mW, 647 nm /150 mW |
| Biomarkers | pan-keratin (K), vimentin (V) |
| Phenotype | K (+) / V (+) / DAPI (+) / α-SMA (-) / CD29 (-) / CD45/CD31 (-) |
| Clusterization status | single |
| Size | Large (>12 µm) |
| Shape | circle |
| Elongation | No |
| Protrusions | No |
| Micronucleus | No |
| Interactions with normal cells | No |
| Interactions with | no |
