Species | human |
Individual | patient |
Cell type | cancer cell |
Cancer type | prostate |
Age | 77 |
Sex | male |
TNM Status | T3N0M0 |
Status at the analysis timepoint | non-metastatic |
Moment of samples collection | preoperative |
Clinical material | peripheral blood |
Collection | EDTA tubes |
Fixative/storage | 4% PFA/-80̊ C |
Protocol | pan-keratin (K) / vimentin (V) / DAPI / α-SMA / CD29 / CD45/CD31 |
acquisition conditions | Objective 40x, low flow, lasers/power: 405 nm/100 mW, 488 nm /100mW, 647 nm /150 mW |
Biomarkers | pan-keratin (K) |
Phenotype | K (+) / V (-) / DAPI (+) / α-SMA (-) / CD29 (-) / CD45/CD31 (-) |
Clusterization status | single |
Size | Large (>12 µm) |
Shape | circle |
Elongation | No |
Protrusions | No |
Micronucleus | No |
Interactions with normal cells | Yes |
Interactions with | platelet(s) |