| Species | Human |
| Individual | Patient |
| Cell type | Cancer cell |
| Cancer type | Prostate |
| Age | 73 |
| Sex | Male |
| TNM Status | T2N0M0 |
| Status at the analysis timepoint | Non-metastatic |
| Moment of samples collection | Preoperative |
| Clinical material | Tumor draining vein blood |
| Collection | EDTA tubes |
| Fixative/storage | 4% PFA/-80̊ C |
| Protocol | Pan-keratin (K) / vimentin (V) / DAPI / α-SMA / CD29 / CD45/CD31 |
| Acquisition conditions | Objective 40x, low flow, lasers/power: 405 nm/100 mW, 488 nm /100mW, 647 nm /150 mW |
| Biomarkers | Pan-keratin (K) |
| Phenotype | K (+) / V (-) / DAPI (+) / α-SMA (-) / CD29 (-) / CD45/CD31 (-) |
| Clusterization status | Single |
| Size | Large (>12 µm) |
| Shape | Circle |
| Elongation | No |
| Protrusions | No |
| Micronucleus | No |
| Interactions with normal cells | Yes |
| Interactions with | Platelet(s) |
