Atlas ID 163

Cell
Category Description
General information
Species Human
Individual Patient
Cell type Cancer cell
Cancer type Colorectal
Clinical information
Age 73
Sex Male
TNM Status TxN2aM0
Status at the analysis timepoint Non-metastatic
Moment of samples collection Preoperative
Material preparation
Clinical material Portal vein blood
Collection EDTA tubes
Fixative/storage 4% PFA/-80̊ C
Protocol Pan-keratin (K) / vimentin (V) / DAPI / α-SMA / CD29 / CD45/CD31
Acquisition conditions Objective 40x, low flow, lasers/power: 405 nm/100 mW, 488 nm /100mW, 647 nm /150 mW
Cell characteristics
Biomarkers Pan-keratin (K), vimentin (V)
Phenotype K (+) / V (+) / DAPI (+) / α-SMA (-) / CD29 (-) / CD45/CD31 (-)
Clusterization status Cluster - homotypic
Size Large (>12 µm)
Shape Circle
Elongation No
Protrusions No
Micronucleus No
Interactions with normal cells Yes
Interactions with Platelet(s)